Ploidy Level and Nuclear DNA Content of <i>Taraxacum shikotanense </i>(<i>Asteraceae</i>)
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- TAKANO Akihito
- Showa Pharmaceutical University
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- NAKANO Mio
- Showa Pharmaceutical University
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- FUNAMOTO Tsuneo
- Showa Pharmaceutical University
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- TAKAHASHI Koko
- Showa Pharmaceutical University
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- YAMANOUCHI Hiroko
- Showa Pharmaceutical University
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- SHINOZAKI Junichi
- Showa Pharmaceutical University
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- INOUE Masashi
- Geopower Drilling Technology School
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- MORITA Tatsuyoshi
- Faculty of Education, Niigata University
Bibliographic Information
- Other Title
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- シコタンタンポポ(キク科)の倍数性と核DNA量
Abstract
<p>To estimate the ploidy level frequencies of Taraxacum shikotanense Kitam. (Asteraceae) in Hokkaido, Japan, the nuclear DNA content (2C) of 292 plants from 13 localities was measured by flow cytometry (FCM) using Petroselinum crispum (2C = 4.5 pg) as the internal standard. The chromosome count of the species showed that 11 plants from seven localities were octoploid (2n = 64 = 8x) and five plants from two localities were nonaploid (2n = 72 = 9x). Monoploid genome sizes (1Cx) of the octoploid plants were 1.14–1.22 pg, which were similar to those of the nonaploid plants (1.13–1.17 pg). The expected 2C value of each ploidy level was calculated based on 1Cx = 1.13–1.22 pg, and the frequency of each ploidy level was evaluated. As a result, 183 plants (62.7%) of T. shikotanense examined by FCM were estimated as octoploid. The hexaploid (6x), heptaploid (7x), nonaploid (9x), and decaploid (10x) plants were putatively detected at low frequencies (0.3–4.1%). The monoploid genome size of T. shikotanense was significantly lower than those of the Japanese diploid species (1.41–1.59 pg). This suggests that T. shikotanense arose from an unknown ancestral species with small genome sizes or experienced DNA downsizing as its ploidy level increased.</p>
Journal
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- The Journal of Japanese Botany
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The Journal of Japanese Botany 98 (1), 19-28, 2023-02-20
The Editorial Board of The Journal of Japanese Botany
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Keywords
Details 詳細情報について
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- CRID
- 1390858131664928640
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- ISSN
- 24366730
- 00222062
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- Text Lang
- ja
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- Data Source
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- JaLC
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- Abstract License Flag
- Allowed