Use of single-protoplast isolates in the study of the mating phenomena of Rhizoctonia solani (Thanatephorus cucumeris) AG-1 IC and IA
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- Qu Ping
- Graduate School of Agricultural Science, Gifu University
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- Aratani Akiko
- Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University
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- Syoji Takako
- Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University
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- Toda Takeshi
- Department of Plant Pathology, North Carolina State University
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- Kubota Mayumi
- Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University
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- Hyakumachi Mitsuro
- Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University
書誌事項
- タイトル別名
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- Use of single-protoplast isolates in the study of the mating phenomena of <i>Rhizoctonia solani</i> (<i>Thanatephorus cucumeris</i>) AG-1 IC and IA
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<p>This study evaluates the effectiveness of using single-protoplast isolates (SPIs) to study the mating phenomena of Rhizoctonia solani AG-1 IC and IA. SPIs obtained from three field isolates (F-1, Rh28, and RO2) of AG-1 IC were paired with representative single-basidiospore isolate (SBI)-M1/-M2 testers, each from their own field isolates, or paired in all possible combinations. Tufts were formed between SPIs and SBI-M1/-M2 testers and between SPIs-M1 and -M2. The separation ratios of SPIs-M1 and -M2 were approximately 1 : 1, which were similar to the results obtained with SBIs. SPIs obtained from three isolates (GNSD, R59, and Tr8) of AG-1 IA, which failed to form basidiospores, were paired in all possible combinations. Although no tufts formed among SPIs from Tr8 and R59, tufts did form between SPIs from GNSD. SPIs from GNSD were separated into homokaryotic (-M1 or -M2) and heterokaryotic isolates, and the separation ratio of -M1 and -M2 was also around 1 : 1. Amplified fragment length polymorphism (AFLP) phenotypes of the tuft isolates formed between GNSD SPIs-M1 and -M2 suggested that these tuft isolates were all heterokaryotic. These results indicate that all three isolates of AG-1 IC and one isolate GNSD of AG-1 IA are heterokaryotic, and that the other two isolates of Tr8 and R59 of AG-1 IA are homokaryotic. Single-protoplast isolates are effective for studies of the mating phenomena of isolates belonging to different AGs of R. solani that could not form a perfect stage.</p>
収録刊行物
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- Mycoscience
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Mycoscience 49 (2), 132-137, 2008
一般社団法人 日本菌学会
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詳細情報 詳細情報について
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- CRID
- 1390858553268939008
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- NII論文ID
- 10021182488
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- NII書誌ID
- AA11003436
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- ISSN
- 16182545
- 13403540
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- NDL書誌ID
- 9450895
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用可