A simple method for isolation of nuclei from <i>Basidiobolus ranarum</i> (Zygomycota)

  • Nakamura Mio
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Nomura Koji
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Abe Jun-ichi P.
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Degawa Yousuke
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Kakishima Makoto
    Graduate School of Life and Environmental Sciences, University of Tsukuba

Bibliographic Information

Other Title
  • A simple method for isolation of nuclei from Basidiobolus ranarum (Zygomycota)

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Abstract

<p>A simple method for isolating the nuclei from Basidiobolus ranarum was established. To improve the yield and purity of nuclei, we investigated maceration methods, buffer composition, and centrifugation conditions to establish an optimal procedure. Basidiobolus ranarum cultured for 5 days was enzymatically macerated and then homogenized and filtrated through stainless steel sieves. The crude cell homogenate was loaded on a layer of buffer containing 50% glycerol and centrifuged at 1500g. The resultant pellet contained pure nuclei.</p>

Journal

  • Mycoscience

    Mycoscience 50 (6), 448-451, 2009

    The Mycological Society of Japan

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