Non-homologous end joining pathway of the human pathogen Cryptococcus neoformans influences homologous integration efficiency but not virulence
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- Li Hao-Man
- Medical Mycology Research Center, Chiba University, Japan
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- Virtudazo Eric V.
- Medical Mycology Research Center, Chiba University, Japan
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- Toh-e Akio
- Medical Mycology Research Center, Chiba University, Japan
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- Yamaguchi Masashi
- Medical Mycology Research Center, Chiba University, Japan
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- Kawamoto Susumu
- Medical Mycology Research Center, Chiba University, Japan
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- Shimizu Kiminori
- Medical Mycology Research Center, Chiba University, Japan
書誌事項
- タイトル別名
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- Non-homologous end joining pathway of the human pathogen <i>Cryptococcus neoformans</i> influences homologous integration efficiency but not virulence
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抄録
<p>The efficiency of gene targeting by integration through homologous recombination (homologous integration, HI) in the human pathogen Cryptococcus neoformans remains unsatisfactory. In order to achieve a much more efficient gene targeting system in C. neoformans, a new double knockout strain in genes involved in the nonhomologous end joining (NHEJ) pathway was constructed. HI frequency was elevated by as much as approximately fivefold in the single or double knockout strains in NHEJ genes, and the frequency depended on the gene targeted. None of the NHEJ gene knockouts showed significant differences in regular growth, sensitivity to DNA-damaging drugs or UV, and virulence compared to the wild-type control, suggesting that the NHEJ pathway does not play a significant role in these biological stresses in C. neoformans. It was also suggested that the genes analyzed in this study are components of a single NHEJ pathway, as the mutants (including the double mutant) displayed the same phenotypes.</p>
収録刊行物
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- Mycoscience
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Mycoscience 51 (4), 272-280, 2010
一般社団法人 日本菌学会
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詳細情報 詳細情報について
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- CRID
- 1390858553269046784
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- NII論文ID
- 10026513911
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- NII書誌ID
- AA11003436
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- ISSN
- 16182545
- 13403540
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- NDL書誌ID
- 10763834
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用可