Reduction of the Side Effects of Photoinduced Cytosolic Dispersion of RNA (PCDR) Based on Photochemical Internalization
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- Bando Akinari
- Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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- Watanabe Kazunori
- Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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- Ohtsuki Takashi
- Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Bibliographic Information
- Other Title
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- Photochemical internalizationに基づく光依存的細胞質内RNA導入法の副作用低減
Abstract
<p>A drawback to carrier-based cytosolic delivery of biomolecules and drugs is that endosomal entrapment of cargoes (biomolecules and drugs) often occurs. Photochemical internalization (PCI) can be used to solve this shortcoming. In PCI, a photosensitizer is encapsulated in the endosome together with the cargo. The photosensitizer can then be photoirradiated to induce endosomal escape and cytoplasmic dispersion of the cargo. This method can deliver the cargo into the cytoplasm with almost no damage to the cells by irradiating with minimum necessary light. However, side effects including cytotoxicity are observed with increasing light intensity. Our recent results suggest that the cause of side effects is “photosensitizers adsorbed on the cell surface,” which are not necessary for PCI. In this study, prior to photoirradiation, we attempted to remove or inactivate photosensitizers on the cell surface by washing the cells, treating with serum, and quenching with trypan blue. We investigated the efficacy of these treatments at reducing the side effects of PCI. We used PCI-based photoinduced cytosolic dispersion of RNA (PCDR) to verify reductions in side effects.</p>
Journal
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- The Journal of Japan Society for Laser Surgery and Medicine
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The Journal of Japan Society for Laser Surgery and Medicine 44 (1), 62-68, 2023-04-15
Japan Society for Laser Surgery and Medicine
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Keywords
Details 詳細情報について
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- CRID
- 1390858752001316608
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- ISSN
- 18811639
- 02886200
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- KAKEN
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- Abstract License Flag
- Disallowed