Fundamental study on the performance of the modified Meixner test to detect the natural toxin α-amanitin

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  • OKADA Kohki
    Department of Medical Technology and Sciences, Faculty of Health Sciences, Kyoto Tachibana University
  • MATSUO Kano
    Graduate School of Health Sciences, Kyoto Tachibana University
  • FUKUDA Atsuhisa
    Department of Medical Technology, Osaka Medical and Pharmaceutical University Mishima-Minami Hospital
  • TAKESHITA Hitoshi
    Department of Medical Technology and Sciences, Faculty of Health Sciences, Kyoto Tachibana University

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  • 改良Meixner試験による自然毒成分α-アマニチンの検出性能に関する基礎的検討
  • カイリョウ Meixner シケン ニ ヨル シゼン ドク セイブンa-アマニチン ノ ケンシュツ セイノウ ニ カンスル キソテキ ケントウ

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Abstract

<p>α-Amanitin (AMA) is primarily a natural toxin component of poisonous mushrooms such as Amanita virosa (AV). In this study, we examined the performance of the Meixner test to detect AMA in various solvents. Although we referred to previous publications, there was a lack of information about the method of the Meixner test, such as the amount and concentration of each reagent. Therefore, in this study, we developed our own procedure named the modified Meixner test. Samples with AMA were prepared in distilled water (DW), urine, and serum. In addition, mushroom component extraction samples were also obtained. As a result of the modified Meixner test performed according to our procedure, a positive reaction was observed in samples with 200 μg/mL AMA in DW. On the other hand, the detection sensitivity decreased when the concentration of hydrochloric acid used in this study was lowered. Positive reactions were also obtained in samples with 200 μg/mL AMA in urine and serum, whereas AMA at concentrations of less than 100 μg/mL was negative in all solvents. Boiling (100°C, 30 min) of each sample did not affect the test results. The sensitivity was decreased in samples prepared in serum and urine to which hemoglobin was added, and AMA at concentrations of less than 200 μg/mL indicated a negative reaction. Three out of five samples with AMA extracted from AV showed a positive reaction with this test. The modified Meixner test showed an AMA detection sensitivity of ≥100 μg/mL, except for hemolytic and hematuria samples.</p>

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