Development of a New Quantitative Method for Polysaccharide by Sandwich Enzyme-Linked Lectin Assay Using Single Lectin Species

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  • OSHIMA Toshifumi
    Analysis Research Department, Food Quality & Safety Research Laboratories R&D Division, Meiji Co., Ltd. Phamaceuticals Dept., Meiji Co., Ltd.
  • NAKAYAMA Kai
    Analysis Research Department, Food Quality & Safety Research Laboratories R&D Division, Meiji Co., Ltd.
  • TAKABAYASHI Kumiko
    Analysis Research Department, Food Quality & Safety Research Laboratories R&D Division, Meiji Co., Ltd.
  • IWAKI Misato
    Analysis Research Department, Food Quality & Safety Research Laboratories R&D Division, Meiji Co., Ltd.
  • UBE Nana
    WORLD INTEC CO., LTD. Yokohama Sales Office
  • NAKAJIMA Akihiro
    Analysis Research Department, Food Quality & Safety Research Laboratories R&D Division, Meiji Co., Ltd.

Bibliographic Information

Other Title
  • 単一レクチン種を用いるサンドイッチEnzyme-Linked Lectin Assayによる高分子多糖類の新規定量技術の開発
  • タンイツ レクチンシュ オ モチイル サンドイッチ Enzyme-Linked Lectin Assay ニ ヨル コウブンシ タトウルイ ノ シンキ テイリョウ ギジュツ ノ カイハツ

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Description

<p>We have developed a new quantitative method for polysaccharide by sandwich enzyme-linked lectin assay (ELLA). In this high specificity method, polysaccharide is reacted with lectin immobilized in a 96-well microtiter plate, and then is sandwiched by enzyme-conjugated lectin, followed by color development by the enzyme activities. Guar gam was detected at a concentration from 0.1 μg mL−1 to 3 μg mL−1, and exopolysaccharide (EPS) from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (R-1) was detected at a concentration from 0.2 μg mL−1 to 1 μg mL−1 by this method. We quantified EPS in the culture fluid of R-1 strain after removing proteins by this method. The values obtained in spike and recovery test were from 97 % to 99 %. The precision of analysis was that repeatability was 6.2 % and intermediate precision was 6.8 %. These results showed this method had enough analytical ability. Furthermore, due to good operability, this method is expected to be applied to the quantification of various polysaccharides.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 72 (4.5), 167-173, 2023-04-05

    The Japan Society for Analytical Chemistry

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