Self-cloning of the Catalase Gene in Environmental Isolates Improves Their Colony-forming Abilities on Agar Media

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  • Watanabe Motoyuki
    Graduate School of Agriculture, Hokkaido University Bioproduction Research Institute, National Institute of Advanced Industrial and Technology (AIST)
  • Igarashi Kensuke
    Graduate School of Global Food Resources, Hokkaido University Bioproduction Research Institute, National Institute of Advanced Industrial and Technology (AIST)
  • Kato Souichiro
    Graduate School of Agriculture, Hokkaido University Bioproduction Research Institute, National Institute of Advanced Industrial and Technology (AIST)
  • Kamagata Yoichi
    Graduate School of Agriculture, Hokkaido University Bioproduction Research Institute, National Institute of Advanced Industrial and Technology (AIST)
  • Kitagawa Wataru
    Graduate School of Agriculture, Hokkaido University Bioproduction Research Institute, National Institute of Advanced Industrial and Technology (AIST)

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<p>Hydrogen peroxide (H2O2) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H2O2 concentration in agar plates. Putative catalase genes, which degrade H2O2, were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under H2O2 pressure. The present results demonstrated high sensitivity to H2O2 even in microbes possessing functional catalase genes.</p>

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