An Alternative Method for Quantitative Mass Spectrometry Imaging (<i>q</i>-MSI) of Dopamine Utilizing Fragments of Animal Tissue

DOI Web Site 参考文献27件
  • Nagano Erika
    Research and Development Division, Miruion Corporation
  • Odake Kazuki
    Research and Development Division, Miruion Corporation
  • Shimma Shuichi
    Research and Development Division, Miruion Corporation Department of Biotechnology, Graduate School of Engineering, Osaka University

抄録

<p>Mass spectrometry imaging (MSI) is a well-known method for the ionization of molecules on tissue sections and the visualization of their localization. Recently, different sample preparation methods and new instruments have been used for MSI, and different molecules are becoming visible. On the other hand, although several quantification methods (q-MSI) have been proposed, there is still room for the development of a simplified procedure. Here, we have attempted to develop a reproducible and reliable quantification method using a calibration curve prepared from tissue debris of a frozen section of a sample when we trim the frozen blocks. We discuss the reproducibility of this method across different sample lots and the effect of the biological matrix (ion suppression) on our results. The quantitative performance was evaluated in terms of accuracy and relative standard deviation, and the reliability of the quantitative values obtained by matrix-assisted laser desorption/ionization-MSI was further evaluated by enzyme-linked immunosorbent assay (ELISA). Our q-MSI method for the quantification of dopamine in mouse brain tissue was found to be highly linear, accurate, and precise. The quantitative values obtained by MSI were found to be highly comparable (>85% similarity) to the results obtained by ELISA from the same tissue extracts.</p>

収録刊行物

  • Mass Spectrometry

    Mass Spectrometry 12 (1), A0128-A0128, 2023-07-25

    一般社団法人　日本質量分析学会

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