Establishment of a manual liquid-based cytology method for uterine cervical cytology, with evaluation of its adequacy for application to the Bethesda System 2014

DOI Web Site
  • SETOGUCHI Chisato
    Department of Pathology and Laboratory Medicine, Omori Red Cross Hospital
  • UMEZAWA Takashi
    Department of Clinical Laboratory Sciences, School of Health Sciences, Fukushima Medical University
  • TATEKAWA Kana
    Department of Laboratory Medicine, Yokohama City Minato Red Cross Hospital
  • YAMAMOTO Yoko
    Department of Laboratory Medicine, Yokohama City Minato Red Cross Hospital
  • HAYASHI Eiichi
    Department of Laboratory Medicine, Yokohama City Minato Red Cross Hospital
  • YAMAMURA Nobukazu
    Department of Laboratory Medicine, Yokohama City Minato Red Cross Hospital
  • KUMAGAI Jiro
    Department of Pathology, Yokohama City Minato Red Cross Hospital

Bibliographic Information

Other Title
  • 子宮頸部擦過細胞診に対する用手法liquid-based cytology導入と適用可能性についての検討
  • シキュウ ケイブ サッカ サイボウシン ニ タイスル ヨウ シュホウ liquid-based cytology ドウニュウ ト テキヨウ カノウセイ ニ ツイテ ノ ケントウ

Search this article

Abstract

<p>Objective: The aim of this study was to establish a manual liquid-based cytology (LBC) method for uterine cervical Papanicolaou preparation, without the need for a specialized automated system. Study Design: 3,383 cervical cytology specimens were examined. Cervex-BrushTM and BD SurePathTM collection vials were used for the specimen collection, and the specimens were processed manually by the manual LBC method. The cytodiagnosis was classified according to the Bethesda System 2014. After the evaluation of the processing and staining of the slides, the specimen adequacy was evaluated and the rate of “unsatisfactory” specimens was analyzed with specification of the reason. The results of cytodiagnosis were then compared with those of the biopsy diagnoses. Results: The quality of the LBC slides processed by the manual LBC method was adequate for making a cytodiagnosis according to the Bethesda System 2014. The cytodiagnoses were as follows: NILM (n = 2,975; 87.9%), ASC-US (n = 148; 4.4%), ASC-H (n = 52; 1.5%), LSIL (n = 119; 3.5%), HSIL (n = 58; 1.7%), and 16 other categories. Among the 130 cases with ASC or worse in which a biopsy was performed, the histological diagnosis was LSIL or HSIL in 99 (76.1%) cases. Conclusion: Manual processing of LBC specimens, without the use of a specialized automated system, could be realized by the manual LBC method, and the slides processed by this system were adequate for cytodiagnosis according to the Bethesda System 2014.</p>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top