Development of a Bioluminescence Detection Method for Endotoxin and Its Application to Dialysate Analysis
-
- YAWATA Satoshi
- DKK-TOA CORPORATION
-
- NODA Kenichi
- DKK-TOA CORPORATION
-
- SHIMOMURA Ai
- DKK-TOA CORPORATION
-
- ODA Atsumu
- DKK-TOA CORPORATION
-
- ARAKAWA Satoshi
- DKK-TOA CORPORATION
-
- HACHIYA Hiromitsu
- DKK-TOA CORPORATION
-
- KURODA Akio
- Graduate School of Integrated Sciences for Life, Hiroshima University
Bibliographic Information
- Other Title
-
- 生物発光式エンドトキシン検出法の開発及び透析液測定への応用
Description
<p>A novel endotoxin detection method was developed that combines a bioluminescence method using a high-luminant mutant luciferase and a lysate reaction. This method reached the detection limit of 0.0005 EU mL−1 in a 15-min duration. A salt-resistant mutant luciferase was developed whose sensitivity was not decreased by sodium chloride (NaCl) in dialysate; it was applied to the endotoxin analyzer for dialysate measurement. It was useful for dialysate measurement with a detection limit of 0.0003 EU mL−1 at 20 min and exhibited other properties under dialysis conditions. A thermostable luciferase was also developed and applied to a single-reaction type endotoxin analyzer. The detection limit was 0.001 EU mL−1 for 10 min, enabling a more rapid endotoxin detection. This novel endotoxin detection method has rapidly been becoming popular in the field of dialysate measurement because of its excellent features.</p>
Journal
-
- BUNSEKI KAGAKU
-
BUNSEKI KAGAKU 72 (7.8), 289-298, 2023-07-05
The Japan Society for Analytical Chemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390860177971277824
-
- ISSN
- 05251931
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- Crossref
-
- Abstract License Flag
- Disallowed