Development of a Bioluminescence Detection Method for Endotoxin and Its Application to Dialysate Analysis

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  • 生物発光式エンドトキシン検出法の開発及び透析液測定への応用

Abstract

<p>A novel endotoxin detection method was developed that combines a bioluminescence method using a high-luminant mutant luciferase and a lysate reaction. This method reached the detection limit of 0.0005 EU mL−1 in a 15-min duration. A salt-resistant mutant luciferase was developed whose sensitivity was not decreased by sodium chloride (NaCl) in dialysate; it was applied to the endotoxin analyzer for dialysate measurement. It was useful for dialysate measurement with a detection limit of 0.0003 EU mL−1 at 20 min and exhibited other properties under dialysis conditions. A thermostable luciferase was also developed and applied to a single-reaction type endotoxin analyzer. The detection limit was 0.001 EU mL−1 for 10 min, enabling a more rapid endotoxin detection. This novel endotoxin detection method has rapidly been becoming popular in the field of dialysate measurement because of its excellent features.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 72 (7.8), 289-298, 2023-07-05

    The Japan Society for Analytical Chemistry

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