Investigation of a Static <i>k</i><sub>L</sub><i>a</i> Measurement Method Using an Optical Dissolved Oxygen Sensor with Fast Response Time

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  • Nagamori Eiji
    Department of Biomedical Engineering, Graduate Course in Applied Chemistry, Environmental and Biomedical Engineering, Osaka Institute of Technology (OIT)
  • Sato Takashi
    Department of Biomedical Engineering, Graduate Course in Applied Chemistry, Environmental and Biomedical Engineering, Osaka Institute of Technology (OIT)

Bibliographic Information

Other Title
  • 応答速度に優れた光学式溶存酸素センサーを用いた静的<i>k</i><sub>L</sub><i>a</i>測定法の検討

Abstract

<p>The kLa value (Oxygen-transfer coefficient) is widely used as a scale-up indicator for culture tanks, and several methods have been used to measure it. Among them, the static method is very simple because it does not require bacteria cultivation, allows flexible changes in measurement conditions, and facilitates repeated measurements. On the other hand, the conventional galvanic cell dissolved oxygen sensor has a significant response delay, making it impossible to evaluate culture conditions with high kLa values such as those used in actual culture. In this study, it was clarified that a high kLa of 300 h−1 or higher can be measured by a static method using an optical dissolved oxygen sensor with a remarkably fast response time. Furthermore, we correlated the results with various kLa measurement methods under exactly the same conditions.</p>

Journal

  • KAGAKU KOGAKU RONBUNSHU

    KAGAKU KOGAKU RONBUNSHU 49 (5), 142-148, 2023-09-20

    The Society of Chemical Engineers, Japan

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