S1PR1受容体を介したマウス歯乳頭由来幹細胞の象牙芽細胞分化

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  • 廣瀨 陽菜
    福岡歯科大・歯・口腔治療学講座・歯科保存学分野
  • 藤政 清志朗
    福岡歯科大・歯・口腔治療学講座・歯科保存学分野
  • 金丸 慎吾
    福岡歯科大・歯・口腔治療学講座・歯科保存学分野
  • 松本 典祥
    福岡歯科大・歯・口腔治療学講座・歯科保存学分野
  • 高橋 富美
    産業医科大・医・薬理学講座
  • 松﨑 英津子
    福岡歯科大・歯・口腔治療学講座・歯科保存学分野 福岡歯科大学・歯・口腔医学研究センター

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  • S1P receptor 1-mediated odontoblastic differentiation of mouse apical papilla-derived stem cells

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<p>Sphingosine-1-phosphate (S1P) is known as a signaling sphingolipid that regulates many cellular responses, including cellular differentiation. We have previously reported that S1P signaling pathway regulates both the promotion of osteoblastogenesis and the inhibition of adipogenesis in C3H10T1/2 cells, which are functionally similar to mesenchymal stem cells. In recent years, the idea of regenerative endodontics has been advocated. The goal of this method is to improve the regenerative capacity by activating stem cells of the apical papilla (SCAPs) present in the apical portion. However, the involvement of S1P signaling in SCAPs differentiation into odontoblast is not well understood. In this study, we investigated the roles of S1PR1-mediated odontoblastic differentiation and mineralization of SCAPs.</p><p>We used the mouse immortalized cell line of SCAPs (iSCAP). iSCAPs expressed S1PR1 and S1PR2, and S1P increased the expression of S1PR1. S1P increased mRNA expression of odontoblastic differentiation marker involving DSPP, DMP-1, and MEPE, and protein secretion of DSPP, DMP-1, which was diminished by inhibitor of S1P receptor 1 (S1PR1). S1P also induced mineralization through S1PR1. We conclude that S1PR1 signaling induces odontoblastic differentiation.</p><p></p>

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