Automated Pulsed Laser Irradiation for Highly Efficient Optoporation

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  • パルスレーザの自動照射による高効率オプトポレーション法の開発

Abstract

<p>Optoporation is a technique for introducing drugs into cells and is expected to be a safe and efficient method because it is a physical technique. However, it has the problem of low throughput due to the irradiation of a single pulse laser to a single irradiation point and the manual switching of filters. These problems have made the search for irradiation conditions inefficient. In this study, we solved these problems by automating filter switching and using continuous laser irradiation. At the same time, we searched optimal laser power by evaluating the molecular incorporation rate and cell viability after laser irradiation.</p>

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