Dormancy breaking of fission yeast spores revealed by establishment of timelapse single cell RNA-seq analyses

DOI
  • Sato Masamitsu
    Department of Life Science and Medical Bioscience, Waseda University

Bibliographic Information

Other Title
  • タイムラプス・シングルセル発現解析の開発による分裂酵母胞子の休眠と発芽の分子機構

Abstract

How cells wake up from dormancy remains enigmatic, although it has long been known that Schizosaccharomyces pombe spores break dormancy in response to glucose uptake from their environment. Expression profiling upon dormancy breaking must be a clue for the question. As RNA-seq profiles made from bulk spores may not display sufficient temporal resolution, we established a system to perform single cell RNA-seq (scRNA-seq) to capture the transcriptional landscape of a single spore upon dormancy breaking. scRNA-seq was performed over 64 single spores in dormancy or germination, and each single-cell transcriptome was mutually compared to predict how transcriptomes changed over time upon dormancy breaking. Thus, each transcriptome was aligned along the 'Virtual Timeline' and a histone H3 gene was highlighted as a differentially expressed gene upon dormancy breaking. The data highlighted a possibility that chromatin dramatically change in accordance with progression of germination.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390862268821051776
  • DOI
    10.60396/iforc.37.0_19
  • ISSN
    27592553
    00738751
  • Text Lang
    ja
  • Data Source
    • JaLC
  • Abstract License Flag
    Disallowed

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