Improved Indophenol Titration Method for Ascorbic Acid Using a Dropper and Electronic Balance: Enhanced Convenience and Efficiency

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  • Umegaki Keizo
    Center for Pharma-Food Research, Graduate School of Pharmaceutical Sciences, University of Shizuoka Faculty of Food and Health Sciences, Showa Women’s University
  • Ito Mika
    Faculty of Food and Health Sciences, Showa Women’s University
  • Yokotani Kaori
    Faculty of Food and Health Sciences, Showa Women’s University
  • Yamada Shizuo
    Center for Pharma-Food Research, Graduate School of Pharmaceutical Sciences, University of Shizuoka

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  • スポイトと電子天びんを用いたアスコルビン酸のインドフェノール滴定法の改良：利便性と効率性の向上

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<p>The analysis of ascorbic acid using the 2,6-dichloroindophenol (DCIP) titration method is a well-established technique, but requires the skilled handling of a burette for accurate measurements. In the present study, we propose a modified DCIP titrimetric method that replaces the burette with a dropper and employs an electronic balance to measure the titrated amount by weight. The dropper used can be flexibly selected, allowing for a wide range of drop sizes, from large to very small. This modification offers several advantages, including lower skill requirements, a 43% reduction in the analysis time, a 50% decrease in sample/reagent consumption, and the ability to prepare DCIP standard solutions tailored to the concentration of ascorbic acid in the sample being analyzed. Our analysis of several food samples using this improved method showed that inherent issues of the DCIP method, such as determining the titration end point, could not be resolved. Nevertheless, the improved titration method remains more convenient and adaptable than the original approach using a burette, enabling quick and accurate analysis, especially for unskilled analysts.</p>

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