In Vivo Spectroscopic Imaging of Caenorhabditis Elegans

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  • MIYAZAKI Shinichi
    1Ph.D. Program in Humanics, University of Tsukuba 2International Institute for Integrative Sleep Medicine
  • HAYASHI v
    1Ph.D. Program in Humanics, University of Tsukuba 2International Institute for Integrative Sleep Medicine 3Graduate School of Medicine, Kyoto University
  • KANO Hideaki
    4Department of Chemistry, Kyushu University

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  • 線虫Caenorhabditis elegans におけるin vivo 分光学的イメージング
  • センチュウ Caenorhabditis elegans ニ オケル in vivo ブンコウガクテキ イメージング

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Abstract

Spectroscopic imaging unveils what kinds of biomolecules exist in cells, tissues, or animals. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging enables us to obtain molecular information in a living model organism, Caenorhabditis elegans. The microspectroscopic system employs a new laser source based on the dual-fiber-output configuration, which generates 50-ps laser pulses with 1 MHz repetition rate. The system enables us to conduct label-free spectroscopic imaging in vivo. Spectroscopic imaging has the potential to uncover crucial questions in life sciences.

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