Development of an Easy-to-Fabricate Micro-Intestine Model for Measuring Trans-Epithelial Electrical Resistance

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  • KODAMA Rei
    Faculty of Science and Technology, Gunma University
  • SATO Kiichi
    Graduate School of Science and Technology, Gunma University

Bibliographic Information

Other Title
  • 経上皮電気抵抗測定のための簡便に作製できるマイクロ腸管モデルの開発

Abstract

<p>Micro-intestine models have attracted attention for studies on intestinal absorption and microbiota. In order to create the intestinal model, it is necessary that monolayer-cultured intestinal epithelial model cells form tight junctions between cells, and to confirm this, measurement of trans-epithelial electrical resistance (TEER) is required. Therefore, we aimed to develop a micro-intestine model for TEER measurement that can be fabricated in a general biochemistry laboratory. We constructed a micro-intestine model by culturing Caco-2 cells as human intestinal epithelial model cells on a microdevice made of polydimethylsiloxane, polyester porous membrane, and platinum wires. The TEER value increased with time as the cells grew, indicating that the developed model is practically useful.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 73 (3), 117-121, 2024-03-05

    The Japan Society for Analytical Chemistry

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