Cloning and Characterization of Protein Fraction Specific of Chlorella vulgaris Endemic East Java

DOI HANDLE オープンアクセス
  • Yanuhar Uun
    Departement of Aquatic Resource Management, Faculty of Fisheries and Marine Science, Universitas Brawijaya
  • Suryanto Heru
    Center of Excellence for Cellulose Composite (CECCom), Department of Mechanical Engineering, Faculty of Engineering, Universitas Negeri Malang
  • Mahasri Gunanti
    Faculty of Fisheries and Marine, Airlangga University
  • Nico Rahman Caesar
    Environmental Science, Postgraduate Programme, Universitas Brawijaya : Doctoral Programme

抄録

Chlorella vulgaris is a type of microalgae that contains active chemical metabolites, including Pigment-Protein Fraction (PPF), which has been shown to possess anti-viral and antiinflammatory properties in fish viral infections. The objective of this study is to characterize PPF of C. vulgaris through cloning and in silico analysis. This study utilized C. vulgaris isolated from the endemic region of East Java, Indonesia. The morphology of the cultured microalgae was observed using a scanning electron microscope (SEM). The PPF was characterized using the SDS-PAGE method, and its cDNA was isolated and analyzed through RT-PCR. Subsequently, the gene was cloned, transformed using E. coli DH5α, and sequenced. The structure and function of PPF were then described through an in-silico procedure. The study revealed that the cultures of C. vulgaris had a high cell density, with a density of 772 x 10^4 cells/mL after being cultured for 7-8 days. The morphology of the microalgae was observed to have a smooth surface with a diameter ranging from 1.5 μm to 2.67 μm. The PPF had a molecular weight of 14 and 35 kDa and its cDNA was visualized using RT-PCR, which revealed a protein with an alkaline pair length of 313 bp. In silico analysis identified two compounds in PPF of C. vulgaris, namely, Succinate CoA ligase and Ammonia form cytochrome c nitrite reductase. These compounds were found to have functions such as protein kinase activity, catalytic activity, and regulation of gene expression.

収録刊行物

  • Evergreen

    Evergreen 11 (1), 82-94, 2024-03

    九州大学グリーンテクノロジー研究教育センター

キーワード

詳細情報 詳細情報について

  • CRID
    1390862776827948032
  • DOI
    10.5109/7172229
  • ISSN
    24325953
    21890420
  • HANDLE
    2324/7172229
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • IRDB
    • Crossref
  • 抄録ライセンスフラグ
    使用可

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