環境DNAを用いた野外水生昆虫・珪藻群集の分析と農薬の生態影響評価への応用

DOI
  • 永井 孝志
    国立研究開発法人農業・食品産業技術総合研究機構農業環境研究部門
  • 稲生 圭哉
    国立研究開発法人農業・食品産業技術総合研究機構農業環境研究部門
  • 横山 淳史
    国立研究開発法人農業・食品産業技術総合研究機構農業環境研究部門

書誌事項

タイトル別名
  • Analysis of aquatic insect and diatom communities using environmental DNA and its application to ecological effect assessment of pesticides

抄録

<p>A methodological investigation was conducted for analyzing the natural communities of aquatic insect and diatom using environmental DNA metabarcoding, followed by the application for ecological effect assessment of pesticides. Water samples were collected from two distinct sites: the Yamaguchi River, as an unpolluted environment, and the Kokai River, characterized by the widespread presence of paddy fields and potentially elevated pesticide concentrations. In consideration of the pesticide effect on riverine ecological communities, the analysis focused on aquatic insect, known to be vulnerable to insecticides, as well as attached diatoms with susceptibility to herbicides. The Yamaguchi River generally exhibited higher values of bioindicators compared to the Kokai River, supporting the promising role of the environmental DNA approach in assessing ecological effect within natural aquatic ecosystems. An advantage of this methodology lies in its capacity for concurrent use alongside environmental monitoring of chemical substances. Ideally, future monitoring should encompass the simultaneous analysis of chemical concentrations and biota.</p>

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390863097404887168
  • DOI
    10.11403/jset.27.32
  • ISSN
    18825958
    13440667
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
  • 抄録ライセンスフラグ
    使用不可

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