Simultaneous quantification of multiple anti-diabetic drugs in whole blood by ultra-performance liquid chromatography-tandem mass spectrometry
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- Suzuki Takayoshi
- Department of Medical Technology, Shubun University Department of Legal Medicine, Aichi Medical University School of Medicine
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- Ogawa Tadashi
- Department of Legal Medicine, Aichi Medical University School of Medicine Poison Analysis Center, Aichi Medical University School of Medicine
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- Iwai Masae
- Department of Legal Medicine, Aichi Medical University School of Medicine Poison Analysis Center, Aichi Medical University School of Medicine
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- Matsuo Tomohito
- Department of Legal Medicine, Aichi Medical University School of Medicine Poison Analysis Center, Aichi Medical University School of Medicine
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- Kondo Fumio
- Department of Legal Medicine, Aichi Medical University School of Medicine Department of Biomedical Sciences, Chubu University College of Life and Health Sciences
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- Seno Hiroshi
- Department of Legal Medicine, Aichi Medical University School of Medicine Poison Analysis Center, Aichi Medical University School of Medicine
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説明
<p>An analytical method was developed and validated for the simultaneous quantification of multiple anti-diabetic drugs in whole blood using ultra-performance liquid chromatography-tandem mass spectrometry. Chromatographic separation was achieved on a CAPCELL PAK INERT ADME-HR column. The mobile phases consisted of water containing 0.1% formic acid and acetonitrile containing 0.1% formic acid. Quantification of 10 antidiabetic drugs was performed by selected reaction monitoring with each product ion referenced against 4 isotopically labelled internal standards. Calibration curves exhibited good linear relationships ranging from 0.0002–20 μg/mL, with correlation coefficients exceeding 0.996. The limits of detection were estimated to be 0.0001–0.005 μg/mL. The intra- and inter-day accuracies and precisions were 98.6–105.5% and 0.1–8.0%, respectively. The recovery efficiencies were in the range of 74.4–102.3%. Matrix effects were observed ranging from -29.1 to 534.6%. Stability tests of the analytes revealed that gliclazide was relatively unstable when stored long-term (28 days) at −30°C. We expect that the method described in the present study will be useful for various studies such as clinical and forensic toxicological investigations.</p>
収録刊行物
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- Medical Mass Spectrometry
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Medical Mass Spectrometry 7 (1), 35-42, 2023-06-25
一般社団法人 日本医用マススペクトル学会
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詳細情報 詳細情報について
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- CRID
- 1390863483180334464
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- NII書誌ID
- AA12788254
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- ISSN
- 2432745X
- 24327441
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- NDL書誌ID
- 032940041
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可