A DUF3844 domain-containing protein is required for vacuolar protein sorting in <i>Schizosaccharomyces pombe</i>

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  • Inagawa Tomoaki
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
  • Ohkubo Kazuma
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
  • Watanabe Masahiro
    Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • Morita Tomotake
    Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • Higuchi Yujiro
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
  • Maekawa Hiromi
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
  • Takegawa Kaoru
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

Description

<p>Protein trafficking to vacuoles in plants and fungi, and to lysosomes in animals, is essential for the maintenance of cellular homeostasis. In Saccharomyces cerevisiae, the vacuolar protein sorting (VPS) pathway has been well studied by using vacuolar carboxypeptidase Y (CPY) as a model, and many VPS genes have been identified. By contrast, the vacuolar protein trafficking pathway in Schizosaccharomyces pombe remains poorly understood. In this study, we identified a novel VPS gene (SPBC1709.03) in S. pombe that is broadly conserved in fungi, but not in S. cerevisiae. Owing to its DUF3844 domain of unknown function, the gene was named vps3844. Disruption mutants of vps3844 had defects in both CPY sorting and incorporation of FM4-64 dye into the vacuolar membrane. Partial deletion analysis of the Vps3844 protein revealed that, within the DUF3844 domain, the region comprising amino acids 354 to 380 is important for protein trafficking to the vacuole. Our findings represent the first report of a VPS gene involved in vacuolar transport that is conserved in fungi, particularly S. pombe, but lacks representation in S. cerevisiae.</p>

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