BASIC RESEARCH ON ESTIMATING ABUNDANCE AND BIOMASS OF UNIONID MUSSELS (<i>Nodularia douglasiae nipponensis</i>) USING ENVIRONMENTAL DNA METHOD

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  • 環境DNA分析を用いたイシガイの生息量評価に関する基礎研究

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<p> The purpose of this study is to obtain the knowledge for establishing the Unionid mussel （Nodularia douglasiae nipponensis） survey method using environmental DNA (eDNA) detection. First of all, the design of the primer probe of the mussel was carried out. Next, laboratory experiments were conducted to evaluate the abundance/biomass of the mussels. As a result, a positive correlation was found between the population of the mussels and the eDNA concentration. In addition, we collected the number of the Unionid mussel collected by hand, the eDNA detection rate and the eDNA concentration in agricultural waterways and small rivers. The survey method using the eDNA detection suggested that it is possible to evaluate the presence or absence of the mussels, and it is possible to identify places with a large number of habitats so that important places for conservation can be identified.</p>

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