Detection of Arcobacter Species in Human Stool Samples by Culture and Real-time PCR
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- YAMAUCHI YUKO
- Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg Department of Infection Control Science, Juntendo University Graduate School of Medicine
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- UEHARA YUKI
- Department of General Medicine, Juntendo University Faculty of Medicine Department of Microbiology, Juntendo University Faculty of Medicine Infection Control Science Research Center, Juntendo University Graduate School of Medicine Department of Clinical Laboratory, St Luke’s International Hospital
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- BOUTIN SÉBASTIEN
- Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg
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- YAMAMOTO NORIO
- Department of Infection Control Science, Juntendo University Graduate School of Medicine Department of Microbiology, Juntendo University Faculty of Medicine
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- KUWAHARA-ARAI KYOKO
- Department of Microbiology, Juntendo University Faculty of Medicine
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- KIRIKAE TERUO
- Department of Microbiology, Juntendo University Faculty of Medicine Infection Control Science Research Center, Juntendo University Graduate School of Medicine
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- HIRAMATSU KEIICHI
- Department of Microbiology, Juntendo University Faculty of Medicine Infection Control Science Research Center, Juntendo University Graduate School of Medicine
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- ZIMMERMANN STEFAN
- Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg
書誌事項
- タイトル別名
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- Detection of <i>Arcobacter</i> Species in Human Stool Samples by Culture and Real-time PCR
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<p>Objective: The clinical significance of Arcobacter species has not been established due to a lack of suitable detection methods.</p><p>Material and Methods: A total of 1,650 stool samples submitted to the Clinical Laboratory of Heidelberg University Hospital were inoculated onto agar plates selective for Campylobacter species isolation and incubated at 37℃.</p><p>Results: Four (0.24%) of the samples were positive for Arcobacter butzleri isolates. Genus-specific primers for real-time PCR were designed to identify Arcobacter species. Of the 1,650 stool samples tested, twelve (0.73%), including the four culture-positive samples, were positive for Arcobacter species by real-time PCR. The sensitivity of real-time PCR was 10 4 CFU g -1 stool, 50 CFU reaction -1 using a stool sample.</p><p>Conclusions: Although the sensitivity of real-time PCR was relatively low compared with other PCR methods, the present method detected a broad range of Arcobacter species. The combination of the stool culture using agar selective for Campylobacter species and real-time PCR for Arcobacter species may be clinically useful for the diagnosis and epidemiology of Arcobacter species infections.</p>
収録刊行物
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- 順天堂醫事雑誌
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順天堂醫事雑誌 66 (5), 431-438, 2020
順天堂医学会
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詳細情報 詳細情報について
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- CRID
- 1391130851449685248
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- NII論文ID
- 130007933296
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- NII書誌ID
- AA1262207X
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- ISSN
- 21882126
- 21879737
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- NDL書誌ID
- 030789217
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可