Evaluation of Conversion Factors for Genetically Modified Maize Quantification
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- Takabatake Reona
- Food Research Institute, NARO
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- Onishi Mari
- FASMAC Co., Ltd.
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- Mano Junichi
- Food Research Institute, NARO
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- Kishine Masahiro
- Food Research Institute, NARO
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- Soga Keisuke
- National Institute of Health Sciences
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- Nakamura Kosuke
- National Institute of Health Sciences
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- Kondo Kazunari
- National Institute of Health Sciences
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- Kitta Kazumi
- Food Research Institute, NARO
Bibliographic Information
- Other Title
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- 遺伝子組換えトウモロコシ定量のための内標比の算出
- イデンシ クミカエ トウモロコシ テイリョウ ノ タメ ノ ナイヒョウヒ ノ サンシュツ
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Abstract
<p>To quantify the amount of authorized GM maize or soybean, conversion factor (Cf) values are required for converting the copy number ratio of GM sequence to an endogenous sequence into weight-based GMO amounts. Cf values are available for the several latest real-time PCR instruments such as QuantStudio5, QuantStudio12K Flex, LightCycler 96, and LightCycler 480 for GM soybeans but not for GM maize. For the quantification of GM maize, we experimentally determined the Cf values targeting Cauliflower mosaic virus 35S promoter (P35S), GA21 construct specific, MIR604 event specific and MIR162 event specific sequences using the four real-time PCR instruments.</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 61 (6), 235-238, 2020-12-25
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1391131406292613504
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- NII Article ID
- 130007960250
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 031241141
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- PubMed
- 33390532
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed