Protein Crystallization under Microgravity and Structure Analysis Using Synchrotron Radiation

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  • KITANO Ken
    Graduate School of Information Science, Nara Institute of Science and Technology )
  • NUMOTO Nobutaka
    Graduate School of Science, Kyoto University
  • MIKI Kunio
    Graduate School of Science, Kyoto University RIKEN Harima ng-8

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Other Title
  • 微小重力下でのタンパク質結晶化と放射光による構造解析
  • ビショウ ジュウリョク カ デ ノ タンパクシツ ケッショウカ ト ホウシャコウ ニ ヨル コウゾウ カイセキ

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Abstract

Protein crystallography is one of the most powerful methods in structural biology to determine the three-dimensional structures of protein molecules at an atomic resolution. However, it is often difficult and laborious to obtain a welldiffracting crystal suitable for structure determination. Since the gravity is one of factors affecting protein crystallization, the microgravity environment has been used to improve the quality of protein crystals. On the other hand, development of the advanced synchrotron facilities such as SPring-8 enabled us to utilize very strong X-ray beams that have almost ideal character for the use in protein crystallography. Here we describe the recent progress of the synchrotron techniques in protein crystallography and discuss the effective crystallization under microgravity for synchrotron experiments.

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