Crystal Growth of a Heme Protein, Cytochrome c′ from Alcaligenes xylosoxidans NCIMB 11015

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  • KOHZUMA Takamitsu
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University National Institute of Advanced Industrial Science and Technology
  • WATANABE Hiroyoshi
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University
  • SATOU Maki
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University
  • NIIZEKI Tomotake
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University
  • UCHIDA Yoshiko
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University
  • TOMOTSUNE Yuko
    Kaken
  • HASHISAKO Fumiaki
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University
  • OBARA Yuji
    Department of Materials and Biological Sciences, Faculty of Science, lbaraki University
  • YOSHIZAKI Izumi
    Japan Aerospace Exploration Agency
  • YAMANAKA Ari
    Japan Space Forum
  • NAKAMURA Hirohiko
    Japan Aerospace Exploration Agency
  • IZUMI Kunihide
    Japan Synchrotron Radiation Research Institute
  • KAJIWARA Kentaro
    Japan Synchrotron Radiation Research Institute
  • ATAKA Mitsuo
    Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology,

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Other Title
  • ヘムタンパク質チトクローム c'の結晶成長
  • ヘムタンパクシツ チトクローム c ノ ケッショウ セイチョウ

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Abstract

The three dimensional structure of protein molecule is essential for understanding numerous cell functions and the industrial utilization of biological systems. It is important to determine the three dimensional structure of biological macromolecule components by certain techniques. Both X-ray and neutron crystallographic techniques require high-quality crystals to obtain precise structural information. Microgravity has so far been expected to give a better condition for protein crystal growth than on the earth. Although physical and chemical approach for the protein crystal growth under the microgravity is strongly believed to be important for the systematic protein crystal design, there are few investigations for the protein crystal growth from the mechanisctic approach of molecular assembly. Cytochromes c' are a class of c-type cytochrome, which have been found in several photosynthetic bacteria and denitrifying bacteria. Cytochrome c' from denytrifying bacteria is a positively charged protein containing a heme prothetic group covalently bound to the protein backborn through two thioether linkages as well as cytochrome c. In the space experiment STS-107, cytochrome c' from Achromobacter xylosoxifdans NCIMB 11015 was used for the crystal growth experiments based on the crystal growth mechanisms. In this paper, a preliminary X-ray topograph analyses of the crystal of cytochrome c' are also provided.

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