Raman Microscopy for Bioimaging

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  • KUMAMOTO Yasuaki
    Nanophotonics Division, Department of Applied Physics, Graduate School of Engineering, Osaka University
  • FUJITA Katsumasa
    Nanophotonics Division, Department of Applied Physics, Graduate School of Engineering, Osaka University

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  • ラマン顕微鏡によるバイオイメージング
  • ラマン ケンビキョウ ニ ヨル バイオイメージング

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Abstract

<p>Raman microscope is not widely used in life sciences. This fact is attributed to the fundamental weaknesses of Raman imaging, i.e., signal weakness and difficulty in molecular identification upon a cell spectrum. Signal weakness leads to long data acquisition time in Raman imaging. We discuss the recent advances in Raman microscopy for accelerating data acquisition speed. Difficulty in spectrum interpretation is attributable to spectral overlapping of vast kinds of molecules existing in a biological sample. We present Raman tags that have characteristic spectra in the silent region, where little intrinsic cellular signal appears. We also present super-resolution techniques that can aid the spectral complexity.</p>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 60 (6), 331-337, 2020

    The Biophysical Society of Japan General Incorporated Association

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