Extrachromosomal transposition of the transposable element Minos in embryos of the cricket Gryllus bimaculatus

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<jats:p>Effective germline transformation of insects has been shown to depend on the right choice of transposon system and selection marker. In this study the promoter region of a <jats:italic>Gryllus cytoplasmic actin</jats:italic> (<jats:italic>GbA3/4</jats:italic>) gene was isolated and characterized, and was used to drive the expression of <jats:italic>Minos</jats:italic> transposase in embryos of the cricket <jats:italic>Gryllus bimaculatus</jats:italic>. Active <jats:italic>Minos</jats:italic> transposase was produced in these embryos as monitored through established transposon excision and interplasmid transposition assays. In contrast, <jats:italic>Drosophila melanogaster hsp70</jats:italic> promoter, previously used to express <jats:italic>Minos</jats:italic> transposase in a number of insect species and insect cell lines, failed to produce any detectable <jats:italic>Minos</jats:italic> transposase activity, as recorded by using the very sensitive transposon excision assay. In addition, the <jats:italic>GbA3/4</jats:italic> promoter was found to drive expression of enhanced green fluorescent protein (eGFP) predominantly in vitellophages of the developing <jats:italic>Gryllus</jats:italic> eggs when a plasmid carrying a <jats:italic>GbA3/4</jats:italic> promoter‐eGFP fusion gene was transiently injected into embryos. These results strongly support the use of <jats:italic>Minos</jats:italic> transposons marked with the <jats:italic>GbA3/4</jats:italic> promoter‐eGFP for the genetic transformation of this emerging model insect species.</jats:p>

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