Cultivation of Yeast Cells by Using n-Alkanes as the Sole Carbon Source : (I) Batch Culture

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  • n-アルカンを炭素源とした酵母培養-1,2-〔英文〕
  • n-アルカン オ タンソゲン ト シタ コウボ バイヨウ 1 , 2 エイブン
  • n-アルカンを炭素源とした酵母培養 : (第1報)回分培養
  • n-アルカンを炭素源とした酵母培養-1,2-〔英文〕

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Abstract

Candida guilliermondii newly isolated from soil was cultivated batchwise in shaken flasks and in a benchi-scale fermentor. A mixture of n-alkanes used principally in this study as the sole carbon source was composed mainly of dodecane (C_<12>) to pentadecane (C_<15>). Specific growth rate μ_x observed in the logarithmic growth phase was about 0.14 hr^<-1>. The value, μ_x≃=0.14 hr^<-1> remained unchanged irrespective of an initial concentration of oil (0.05 to 5.0 vol %) at time t=0.Maximum cell concentration obtained in each run was nearly proportional to the initial oil concentration, provided the concentration was lower than 1.0 vol %. Yield factor Y_<x/s> evaluated from the logarithmic growth of the cells was nearly 0.70.No marked difference was noted in the effect of n-paraffinic carbon numbers on the cell growth except that the cell concentration ever attained by using pure n-tridecane (C_<13>) was a little lower than those from pure n-dodecane (C_<12>) and n-tetradecane (C_<14>).Specific growth rate deteriorated in this work by adding a small amount of surfactant in the culture medium. The addition of surfactant caused an appreciable separation of cells from the oil-medium interface. Cells were adsorbed most likely at the interface in the absence of the surfactant.A favorable contact in the cultivation between cells and oil droplets seemed indispensable for expecting a normal pattern of the cell growth.

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