Tryptic Soy agar supplemented with bacitracin represents a remarkable advancement as a selective culture medium for Streptococcus anginosus

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  • Fukuda Yu
    Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine
  • Imai Susumu
    Department of Translational Research, Tsurumi University School of Dental Medicine
  • Hamada Yoshiki
    Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine
  • Hanada Nobuhiro
    Department of Translational Research, Tsurumi University School of Dental Medicine

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  • Tryptic Soy agar supplemented with bacitracin represents a remarkable advancement as a selective culture medium for <i>Streptococcus anginosus</i>

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<p>Purpose: To develop a new selective culture medium specifically for Streptococcus anginosus.</p><p>Materials and Methods: Three antibiotics, sulphamethazine (s), aztreonam (a), and bacitracin (b), were tested by adding to Tryptic Soy (TS) agar at different concentrations. Initially, sulphamethazine (1.0 mg/mL) and aztreonam (0.2 mg/mL) were added to TS agar to obtain TS-sa agar. In total, 24 strains of oral bacteria including three clinical S. anginosus isolates were used during the entire study. A minimal inhibitory concentration (MIC) test was performed by using 96-well plate to determine the optimal bacitracin concentration for the selective medium for S. anginosus under anaerobic condition at 37ºC for 48 hours. Finally, bacitracin was added at a concentration of 2.0 U/mL to TS-sa agar to prepare TS-sab agar medium and selectivity was tested using all 24 strains of bacteria.</p><p>Results: Most strains of S. anginosus could grow on the TS-sab agar medium but other bacteria except for S. intermedius and S. sobrinus could not be detected on this medium. S. intermedius and S. sobrinus occasionally showed some growth on TS-sab agar medium with low detection rate. Although some clinical isolates of S. anginosus were not enriched like other strains of S. anginosus, a remarkable enhancement was achieved in this study in the development of a selective culture medium for S. anginosus.</p><p>Conclusion: TS-sab may serve as a remarkably improved semi-selective culture medium for S. anginosus. Continued studies are required to improve the selectivity specific for S. anginosus.</p>

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