Cloning and Nucleotide Sequencing of Genes Encoding Mn-Superoxide Dismutase and Class 2 Fumarase from Thermus aquaticus YT-1
書誌事項
- タイトル別名
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- Cloning and Nucleotide Sequencing of Ge
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説明
Abstract A 6955-bp sequence of a Pst I- Hin dIII DNA fragment containing the manganese-superoxide dismutase (MnSOD) gene of Thermus aquaticus YT-1 was determined. The gene ( sod ) encoded a polypeptide consisting of 204 amino acid residues (the mature enzyme without the initiation methionine) with a calculated Mr of 22,773. The deduced amino acid sequence of the sod gene showed 92% identity to that of Thermus thermophilus HB8 determined chemically. The sod gene was well expressed in Escherichia coli and a heat-stable active enzyme was produced. An open reading frame encoding fumarase was found 91 bp upstream of the MnSOD gene in tandem form. The fum gene encoded a polypeptide consisting of 466 amino acid residues with a calculated M r of 50,950. The deduced amino acid sequence of the fum gene product has similarity to that of the fumC of E. coli (57% identity), suggesting that this gene encodes a class II type fumarase.
収録刊行物
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- Journal of Fermentation and Bioengineering
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Journal of Fermentation and Bioengineering 86 (1), 21-27, 1998-07
Suita : Society of Fermentation and Bioengineering
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詳細情報 詳細情報について
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- CRID
- 1520291855736380288
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- NII論文ID
- 110002684096
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- NII書誌ID
- AA10702688
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- ISSN
- 0922338X
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- NDL書誌ID
- 4538437
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
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- データソース種別
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