Microintaglio printing of biomolecules and its application to in situ production of messenger ribonucleic acid display microarray
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説明
A new molecular printing technology for a simple and robust patterning of biomolecules was developed and applied to producing a genotype-linked protein microarray. A microarray of messenger ribonucleic acids (mRNAs) encoding the green fluorescent protein was patterned by microintaglio printing using a micromold comprising an array of uniformly arranged 5-µm-diameter holes at a density of 10,000 per mm2. Furthermore, one-step conversion from an mRNA microarray into an mRNA-protein fusion microarray was performed by simultaneous cell-free protein synthesis and fusion reaction using a puromycin-labeled oligonucleotide linker. The set of developed technologies provides a powerful means of in vitro protein evolution.
収録刊行物
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- Applied physics express : APEX
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Applied physics express : APEX 4 (4), 047001-, 2011-04
Tokyo : Japan Society of Applied Physics
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詳細情報 詳細情報について
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- CRID
- 1520573329790764672
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- NII論文ID
- 10028210017
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- NII書誌ID
- AA12295133
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- ISSN
- 18820778
- 18820786
- http://id.crossref.org/issn/18820786
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- NDL書誌ID
- 11075016
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZM35(科学技術--物理学)
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- データソース種別
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- NDLサーチ
- Crossref
- CiNii Articles
- OpenAIRE
