Photon Counting Histogram Using Numerical Data of Point Spread Function
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<jats:p> Photon counting histogram (PCH) is a fluorescence fluctuation method that can quantify the brightness and concentration of different fluorescent molecules in solution at a single molecule level using confocal optics. The current method assumes that the point spread function (PSF) of confocal optics is Gaussian function. However, this could be problematic when the actual PSF profile is not Gaussian. Here, we propose an improved PCH analysis method using numerical PSF data. This method does not require any analytical equation for the PSF profile, and can handle any arbitrary shape of PSF. We report a proof-of-concept estimation of this method using a model PSF. </jats:p>
収録刊行物
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- Japanese journal of applied physics : JJAP
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Japanese journal of applied physics : JJAP 52 (3), 038001-, 2013-03
Tokyo : The Japan Society of Applied Physics
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詳細情報 詳細情報について
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- CRID
- 1520853832556080512
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- NII論文ID
- 40019617172
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- NII書誌ID
- AA12295836
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- ISSN
- 00214922
- 13474065
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- NDL書誌ID
- 024358177
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZM35(科学技術--物理学)
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- データソース種別
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- NDL
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