Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan
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抄録
<jats:title>ABSTRACT</jats:title><jats:sec><jats:label /><jats:p>Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2‐fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.</jats:p></jats:sec>
収録刊行物
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- Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research
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Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research 62 (10), 673-676, 2018-10
Richmond : Blackwell Publishing Asia Pty
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詳細情報 詳細情報について
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- CRID
- 1522543655090228096
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- NII論文ID
- 40021702629
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- NII書誌ID
- AA00738350
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- ISSN
- 03855600
- 13480421
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- NDL書誌ID
- 029304758
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
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- データソース種別
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- NDL
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