A simple method for isolation of nuclei from Basidiobolus ranarum (Zygomycota)
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- Nakamura Mio
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Nomura Koji
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Abe Jun-ichi P.
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Degawa Yousuke
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- Kakishima Makoto
- Graduate School of Life and Environmental Sciences, University of Tsukuba
書誌事項
- タイトル別名
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- A simple method for isolation of nuclei from <i>Basidiobolus ranarum</i> (Zygomycota)
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説明
<p>A simple method for isolating the nuclei from Basidiobolus ranarum was established. To improve the yield and purity of nuclei, we investigated maceration methods, buffer composition, and centrifugation conditions to establish an optimal procedure. Basidiobolus ranarum cultured for 5 days was enzymatically macerated and then homogenized and filtrated through stainless steel sieves. The crude cell homogenate was loaded on a layer of buffer containing 50% glycerol and centrifuged at 1500g. The resultant pellet contained pure nuclei.</p>
収録刊行物
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- Mycoscience
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Mycoscience 50 (6), 448-451, 2009
一般社団法人 日本菌学会
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詳細情報 詳細情報について
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- CRID
- 1390858553269043712
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- NII論文ID
- 10027042818
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- NII書誌ID
- AA11003436
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- ISSN
- 16182545
- 13403540
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- NDL書誌ID
- 10441399
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用可