Bordetella effector BopN is translocated into host cells via its N-terminal residues
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<jats:title>ABSTRACT</jats:title><jats:sec><jats:label /><jats:p><jats:italic>Bordetella bronchiseptica</jats:italic> infects a wide variety of mammals, and the type III secretion system (T3SS) is involved in long‐term colonization by <jats:italic>Bordetella</jats:italic> in the trachea and lung. T3SS translocates virulence factors (commonly referred to as effectors) into host cells, leading to alterations in the host's physiological function. The <jats:italic>Bordetella</jats:italic> effectors BopN and BteA are known to have roles in up‐regulation of IL‐10 and cytotoxicity, respectively. Nevertheless, the mechanism by which BopN is translocated into host cells has not been examined in sufficient detail. Therefore, to determine the precise mechanisms of the BopN translocation into host cells, we built truncated derivatives of BopN and evaluated the derivatives’ ability to translocation into host cells by adenylate cyclase‐mediated translocation assay. It was found that N‐terminal amino acid (aa) residues 1–200 of BopN are sufficient for its translocation into host cells. Interestingly, BopN translocation was completely blocked by deletion of the N‐terminal aa residues 6–50, indicating that the N‐terminal region is critical for BopN translocation. Furthermore, BopN appears to play an auxiliary role in BteA‐mediated cytotoxicity. Thus, BopN can apparently translocate into host cells and may facilitate activity of BteA.</jats:p></jats:sec>
収録刊行物
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- Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research
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Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Host Defense Research 61 (6), 206-214, 2017-06
Richmond : Blackwell Publishing Asia Pty
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詳細情報 詳細情報について
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- CRID
- 1523106605344189952
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- NII論文ID
- 40021227525
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- NII書誌ID
- AA00738350
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- ISSN
- 03855600
- 13480421
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- NDL書誌ID
- 028301901
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
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- データソース種別
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- NDL
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