DNA Sequence of the β-Isopropylmalate Dehydrogenase Gene and Phylogenetic Analysis of the Yeast Saccharomyces exiguus Yp74L-3

DOI Web Site Web Site Web Site PubMed 被引用文献1件 参考文献54件

書誌事項

タイトル別名
  • DNA Sequence of the b Isopropylmalate Dehydrogenase Gene and Phylogenetic Analysis of the Yeast Saccharomyces exiguus Yp74L-3

この論文をさがす

説明

The beta-isopropylmalate dehydrogenase (LEU2) gene from a homothallic wild-type yeast, Saccharomyces exiguus Yp74L-3, was analyzed to estimate the phylogenetic position of this strain in yeasts. The beta-isopropylmalate dehydrogenase gene of Yp74L-3 was first isolated as a clone complementing the leu2 mutation of Saccharomyces cerevisiae, and then confirmed to complement the haploid leu2 mutant derived from strain Yp74L-3 through genetic transformation. The nucleotide sequence of the cloned DNA revealed an open reading frame (ORF) encoding the beta-isopropylmalate dehydrogenase composed of 365 amino acids. The beta-isopropylmalate dehydrogenase coding sequence from the Yp74L-3 strain displayed 76.7% similarity to that of S. cerevisiae. Candidates for a UAS and a TATA-box in the 5'-upstream region and for a poly-A attachment site in the 3'-downstream region were found. A phylogenetic tree constructed from the nucleotide sequences of the beta-isopropylmalate dehydrogenase coding regions revealed that Yp74L-3 is located between S. cerevisiae and the Kluyveromyces yeasts. The LEU2 gene cloned from Yp74L-3 will serve as an effective genetic marker for constructing the transformation system in S. exiguus Yp74L-3.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (54)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ