Detection of Flaviviruses by Reverse Transcriptase-Polymerase Chain Reaction with the Universal Primer Set
書誌事項
- タイトル別名
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- Detection of Flaviviruses by Reverse Tr
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<jats:title>Abstract</jats:title><jats:p>Using a universal primer set designed to match the sequence of the NS1 gene of flaviviruses, the virus RNA of dengue (DEN), Japanese encephalitis (JEV), powassan and langat of Flaviviridae were successfully amplified by polymerase chain reaction (PCR) via cDNA; and with different internal primers, the serotypes of the dengue viruses were identified. Of the 78 clinically diagnosed dengue fever patients, 18 patients were positive for DEN 1, 48 patients for DEN 2 and 8 patients concurrently infected with DEN 4. Of the 52 patients admitted with Japanese encephalitis (JE), 45 were determined to be JEV infections. By nested PCR, we completed the identification of flaviviruses within 2 days. The results show that seven primers have a potential value for rapid clinical diagnosis of flavivirus infections.</jats:p>
収録刊行物
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- Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Immunology
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Microbiology and immunology / Japanese Society for Bacteriology, Japanese Society for Virology, Japanese Society for Immunology 41 (3), 209-213, 1997-03
Tokyo : Center for Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1523951030016005504
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- NII論文ID
- 130003484490
- 10004896057
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- NII書誌ID
- AA00738350
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- ISSN
- 03855600
- 13480421
- http://id.crossref.org/issn/03855600
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- NDL書誌ID
- 4163583
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- Web Site
- http://id.ndl.go.jp/bib/4163583
- https://ndlsearch.ndl.go.jp/books/R000000004-I4163583
- https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1348-0421.1997.tb01192.x
- https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1348-0421.1997.tb01192.x
- https://search.jamas.or.jp/link/ui/1997188313
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
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- データソース種別
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- NDL
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