Enzymatic pathway for biodegrading microcystin LR in Sphingopyxis sp. C-1
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説明
The mlr gene cluster consisting of mlrA, mlrB, mlrC, and mlrD is involved in the degradation of the cyanobacterial toxin microcystin. However, it is unclear which degradation intermediates are metabolized by MlrB and MlrC. To address these questions, we constructed recombinant Escherichia coli to overproduce MlrB and MlrC from Sphingopyxis sp. C-1, and determined which intermediates were degraded in cell-free extracts. The cell-free extract containing MlrB degraded linearized microcystin-LR, giving rise to a tetrapeptide. The cell-free extract of MlrC degraded linearized microcystin-LR and also degraded the tetrapeptide to the amino acid Adda. These results indicate that linearized microcystin-LR is degraded by both MlrB and MlrC, and tetrapeptide is degraded by specifically by MlrC in Sphingopyxis sp. C-1.
収録刊行物
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- Journal of bioscience and bioengineering
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Journal of bioscience and bioengineering 114 (6), 630-634, 2012-12
Suita : Society for Biotechnology
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詳細情報 詳細情報について
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- CRID
- 1524232505317751808
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- NII論文ID
- 110009578157
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- NII書誌ID
- AA11307678
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- ISSN
- 13891723
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- NDL書誌ID
- 024167049
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- PubMed
- 22883536
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- 本文言語コード
- en
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- 資料種別
- journal article
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- NDL 雑誌分類
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- ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
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- データソース種別
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- NDLサーチ
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