A plant-specific DYRK kinase DYRKP coordinates cell morphology in Marchantia polymorpha
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<jats:title>Abstract</jats:title><jats:p>Dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs) are activated via the auto-phosphorylation of conserved tyrosine residues in their activation loop during protein translation, and they then phosphorylate serine/threonine residues on substrates. The DYRK family is widely conserved in eukaryotes and is composed of six subgroups. In plant lineages, DYRK homologs are classified into four subgroups, DYRK2s, yet another kinase1s, pre-mRNA processing factor 4 kinases, and DYRKPs. Only the DYRKP subgroup is plant-specific and has been identified in a wide array of plant lineages, including land plants and green algae. It has been suggested that in <jats:italic>Arabidopsis thaliana</jats:italic> DYRKPs are involved in the regulation of centripetal nuclear positioning induced by dark light conditions. However, the molecular functions, such as kinase activity and the developmental and physiological roles of DYRKPs are poorly understood. Here, we focused on a sole DYRKP ortholog in the model bryophyte, <jats:italic>Marchantia polymorpha</jats:italic>, MpDYRKP. MpDYRKP has a highly conserved kinase domain located in the C-terminal region and shares common sequence motifs in the N-terminal region with other DYRKP members. To identify the roles of MpDYRKP in <jats:italic>M. polymorpha</jats:italic>, we generated loss-of-function Mp<jats:italic>dyrkp</jats:italic> mutants via genome editing. Mp<jats:italic>dyrkp</jats:italic> mutants exhibited abnormal, shrunken morphologies with less flattening in their vegetative plant bodies, thalli, and male reproductive organs, antheridial receptacles. The surfaces of the thalli in the Mp<jats:italic>dyrkp</jats:italic> mutants appeared uneven and disordered. Moreover, their epidermal cells were drastically altered to a narrower shape when compared to the wild type. These results suggest that MpDYRKP acts as a morphological regulator, which contributes to orderly tissue morphogenesis via the regulation of cell shape.</jats:p>
収録刊行物
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- Journal of plant research / Botanical Society of Japan
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Journal of plant research / Botanical Society of Japan 134 (6), 1265-1277, 2021-11
[Tokyo] : [Springer Japan]
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詳細情報 詳細情報について
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- CRID
- 1524232505980423424
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- NII論文ID
- 40022725207
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- NII書誌ID
- AA10900218
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- ISSN
- 09189440
- 16180860
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- NDL書誌ID
- 031771083
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZR3(科学技術--生物学--植物)
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- データソース種別
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- NDL
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