Production of Tannase and Degradation of Chestnut Tannin by Bacteria :

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The production of tannase (tannin acyl-hydrolase) was demonstrated in cultivated strains of Bacillus pumilus, B. polymyxa, Corynebacterium sp., and Klebsiella pneumoniae, with chestnut bark extract as the sole carbon source. The rapid degradation of the tannin structure was related to the production of extracellular tannase assayed by a new method. Gallic acid was the only degradation product observed with 3 of the strains, but intermediate products were detected in the cultivation broth of Bacillus pumilus. The optimum pH of enzyme activity was 5.5.

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