Cell Biological Characteristics of a Human Embryonal Carcinoma Cell Line
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- YAMAZAWA,Koji
- Department of Obstetrics and Gynecology, Chiba University School of Medicine
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- SEKIYA,Souei
- Department of Obstetrics and Gynecology, Chiba University School of Medicine
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- KIMURA,Hiroaki
- Department of Obstetrics and Gynecology, Chiba University School of Medicine
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- KAWATA,Makoto
- Department of Obstetrics and Gynecology, Chiba University School of Medicine
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- INABA,Noriyuki
- Department of Obstetrics and Gynecology, Chiba University School of Medicine
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- TAKAMIZAWA,Hiroyoshi
- Department of Obstetrics and Gynecology, Chiba University School of Medicine
Bibliographic Information
- Other Title
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- ヒトEmbryonal carcinoma培養細胞株の細胞生物学的特徴
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Abstract
We examined the cell biological characteristics of a human embryonal carcinoma cell line, NEC l4, derived from a male germ cell tumor of testis origin. The following results were obtained. 1) The morphological characteristics of NEC 14 cells were small size, an increase in the N/C ratio and poor development of organelle and desmosome-like cell-cell junctions. The NEC 14 cells proliferated rapidly and the population doubling time in vitro was 17 hours. 2) The functional characteristics of NEC 14 cells were the localization of intermediate filaments such as keratin and vimentin, hCG secretion and tissue plasminogen activator and laminin synthesis and expression of cell surface antigens such as stage-specific embryonic antigen-3, trophoblast antigen and neuron cell surface antigen. 3) The mode of chromosome number was 58 and many abnormal and undetermined chromosomes were found in the NEC 14 cells. 4) Differentiation was observed in vitro and in the tumor tissues xenotransplanted into nude mice.
Journal
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- 日本産科婦人科學會雜誌
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日本産科婦人科學會雜誌 42 (1), 53-59, 1990-01-01
日本産科婦人科学会
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Details 詳細情報について
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- CRID
- 1541698620240968192
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- NII Article ID
- 110002105448
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- NII Book ID
- AN00190060
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- Text Lang
- ja
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- Data Source
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- NDL-Digital
- CiNii Articles