Purification and Properties of Cellulases from Eupencillium javanicum : Studies on the Re-utilization of Cellulosic Resources(VII) :

NDLデジタルコレクション
  • TANAKA,MITSUO
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • TANIGUCHI,MIYAYUKI
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • MATSUNO,RYUICHI
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • KAMIKUBO,TADASHI
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

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説明

Five cellulases[EC3.2.1.4], A-4-E, B-1-E, B-4-E, B-5-E and B-7-E, were fractionated by column chromatography and polyacrylamide gel disc electrophoresis from a crude cellulase preparation derived from the culture filtrate of a fungus, Eupenicillium javanicum.Each cellulase showed a single band on disc electrophoresis. The molecular weights of the five cellulases were estimated to be about 21,000,65,000,47,000,41,000 and 30,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The optimum temperature was about 55℃, and the optimum pH values for carboxymethyl cellulose and crystalline cellulose degradations were 5.0 and 5.5,respectively, in all cellulases. The cellulase A-4-E was less stable to heat treatment than the other coimponents. All cellulases were stable for 24 hr at 3℃ and for 2 hr at 40℃ in the ranges of pH 4-7 and 4-6,respectively. The combinations of cellulases B-4-E and B-5-E and cellulases B-5-E and B-1-E showed strong synergistic action in the solubilization of crystaline cellulose, whereas the combinations of cellulases B-4-E and B-1-E, B-1-E, A-4-E and B-5-E, and A-4-E and B-1-E, showed virtually no synergistic action. The combination of all cellulases showed the strongest synergistic action.

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