市販抗生物質の耐性ブドウ球菌に有効なる抗生物質の研究 : (第2報)耐性ブドウ球菌に抗菌力を有する放線菌35NT号菌の生産する抗生物質について

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  • Studies on an Effective Antibiotic Against Staphylococcus aureus Resistant to Commercial Antibiotics : (II) A New Antibiotic Produced by Streptomyces 35NT Strain Effective Against Resistant Staphylococcus aureus

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Strain No. 35NT of Streptomyces isolated from soil produces a new antibiotic which shown antibiotic activity against Staphyloococcus aureus which is resistant to commercial antibiotics.Strain No. 35NT has morphological and cultural characteristics resembling Streptomyces celluloflavus. By using a submerged culture in a suitable medium containing 1% glucose, 0.5% soybean meal, 0.5% peptone, 0.3% CaCO_3,0.5% NaCl, a No. 35NT substance was obtained.For example, 750μg/ml of antibiotic activity was obtained after 45hrs, using a 30 liter fermentor having an agitator speed of 300rpm and aerated at a rate of 1 vvm.The crude antibiotic was extracted from filtrate of broth by adsorption on active carbon followed by acidic methanol elution.Powder of the antibiotic were obtained by precipitating with (NH_4)_2SO_4,eluting with pyridine and reprecipitating with ethyl ether. It is a slightly yellowish powder containing the nitro group and pentose. The molecular formula is C_14H_24N_2O_10,and it decomposes at 238-240℃.It is active against Gram-positive bacteria only, and inhibits growth of resistant Staphylococcus aureus at a concentration of 0.5μg/ml.

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