Gas Chromatographic-mass Spectrometric Analysis of Gases Produced by Trichomonas Vaginalis in vitro

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  • ISHIGURO,Tatsuya
    Department of Obstetrics and Gynecology, Shiga University of Medecal Science

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  • 膣トリコモナス産生ガスのGC-MSによる成分分析

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Five strains of Trichomonas vaginalis were independently inoculated into Cysteine-Peptone-Liver infusion-Maltose medium (CPLM medium), and gases in the culture bottle were qualitatively analysed at five days after the inoculation by gaschromatography mass spectrometry (GC-MS). Methane, ethane and carbon dioxide were commonly found in the gases produced during the culture of Trichomonas vaginalis in vitro. Carbon monoxide was also found in the gases from 4 of 5 strains. Small amunt of both ethylene and propane was also noted in 3 of 5 strains. These gaseous molecules were supposesd to be produced by Trichomonas vaginalis in vitro, presumably by the catabolism of amino acids. It was also suggested that responsible for the foamy discharge found in the vatina of patients with Trichomonas vaginalis.

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