Fundamental Studies on Differentiation Inducers Using a Human Embryonal Carcinoma Model

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  • YAMAZAWA,Koji
    Department of Obstetrics and Gynecology, Chiba University School of Medicine
  • SEKIYA,Souei
    Department of Obstetrics and Gynecology, Chiba University School of Medicine
  • KIMURA,Hiroaki
    Department of Obstetrics and Gynecology, Chiba University School of Medicine
  • SHIRASAWA,Hiroshi
    Department of Obstetrics and Gynecology, Chiba University School of Medicine
  • KAWATA,Makoto
    Department of Obstetrics and Gynecology, Chiba University School of Medicine
  • TAKAMIZAWA,Hiroyoshi
    Department of Obstetrics and Gynecology, Chiba University School of Medicine

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Other Title
  • ヒトEmbryonal Carcinoma細胞モデルを用いた分化誘導療法に関する基礎的研究

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Abstract

A human embryonal carcinoma (EC) cell line, NEC14, was pluripotent and the tumors formed in nude mice contained differentiated somatic and extra-embryonic elements in addition to EC. The NEC14 cells could be induced to differentiate with 5 differentiation inducers in vitro, and N, N'-hexamethylene bisacetamide (HMBA) showed the most potent differentiator induction among them. All of the NEC14 cells were morphologically differentiated when exposured to 10^<-2>M HMBA for 3 days. The differentiated derivatives ceased to proliferatein vitro and became non-tumorigenic in nude mouse. Several differentiation markers appeared in the differentiated derivatives. When the tumor-bearing nude mice were injected intraperitoneally with HMBA (10mg, twice a week), the tumor growth was inhibited. These results show that human EC is a useful model of differentiation-induction in cancer therapy.

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