Degradation of Yeast Ribonucleic Acid by Polynucleotide Phosphorylase from <i>Azotobacter agilis</i> (<i>vinelandii</i>):I. General Features of the Processes of Phosphorolysis and Arsenolysis

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A modified method for preparing 170-fold purified polynucleotide phosphorylase [EC 2. 7. 7. 8] from Azotobacter agilis and general features of the processes of phosphorolysis and arsenolysis of highly polymerized yeast RNA were described. The products of the above reactions were four nucleoside 5'-diphosphates and nucleoside 5'-monophosphates, respectively.<br> Similar results concerning the effect of substrates and KCl concentration and a little different results in respect to the effect of MgCl2, optimal pH, degradation velosity at optimal condition were obtained with the two reactions. MnCl2 can replace MgCl2 as a cofacter. A higher activity (2.7 times) of MnCl2 than MgCl2 was observed at a low concentration (0.5mM).

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