X-Irradiation Activates the Drosophila 1731 Retrotransposon LTR and Stimulates Secretion of an Extracellular Factor That Induces the 1731-LTR Transcription in Nonirradiated Cells.

J-STAGE

この論文をさがす

説明

Using constructs expressing the reporter gene under the control of the entire or deleted long terminal repeats (LTRs) of 1731, a Drosophila melanogaster retrotransposable element, we show that 1731-LTR is activated by X-irradiation in a dose- and time-dependent manner, and that a sequence located in the U3 region of these LTRs is required. The cis-acting element conferring X-responsiveness shows similarities to kappaB (κB)-like binding sequence. In response to X-irradiation, S2 Drosophila cells produced an extracellular factor which activates the 1731-LTR in nonirradiated cells. This factor was detected both when transfected cells were cocultured with inducing cells and when a conditioned medium taken from irradiated cultures was added.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ