Studies on the Gamma Ray Irradiation on Protein:I. Effect on the Sulfhydryl Groups, Polarographic Patterns and Electron Spin Resonance Absorption of Bovine Serum Albumin
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- IMAI YASUO
- the Department of Physiology, Faculty of Medicine, University of Kyoto
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説明
1. Using a Co60 gamma ray source (intensity 3×103r per hours), the effects of irradiation on bovine serum albumin in dissolved or solid state were studied with amperometric titration of-SH groups, polarographic protein wave recording and paramagnetic resonance absorption spectroscopy.<br> 2. The content of -SH groups was de-creased when a protein solution was irradiated, especially in the presence of air, while hardly any reduction was observed on the solid samples in the absence of air. Such a decrease of-SH groups would result from oxidation. Increase in the amount of-SH groups observed on the solid samples irradi-ated in the presence of air could be attribu-table to the secondary configurational changes of molecules.<br> 3. Marked changes of polarographic patterns of protein double waves were observed on both dissolved and solid samples and their time course or correlation with dose of radiation was studied.<br> These changes in protein waves did not always run parallel with those in the content of titrable -SH groups.<br> 4. Formation of free radicals in the protein molecules by gamma ray irradiation could be proved with ESR absorption studies.<br> The author wishes to express his cordial thanks to Prof. A. Inouye (Department of Physiology, Faculty of Medicine, Kyoto University) and Assistant Prof. T. Hashi (Department of Physics, Faculty of Science, Kyoto University) for their helpful advice and discussions. The author also wishes to express his appreciation to Dr. A. Hirai and Mr. K. Sugibuchi of Department of Physics for their valuable assistance in the ESR studies and to Mr. Y. Naka-yama (Institute for Chemical Research, Kyoto University) for his technical help in gamma ray irradiation.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 49 (2), 85-90, 1961
The Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1570009753220758400
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- NII論文ID
- 130003536624
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles